Categories Select Category Academic Journal of Second Military Medical University Acta Academiae Med

Objective The anti-inflammatory effects of tulathromycin were studied. doggy diapers Method doggy diapers In vitro, we make use of the model that is LPS-induced porcine peripheral blood mononuclear cells (PBMC) overexpressing pro-inflammation factors was used, and then the real-time doggy diapers Q-PCR was employed to study the effect of different concentrations of tulathromycin and erythromycin on the generation of NO and PGE2 involved in the inflammatory process on the molecular level. Result Results showed that tulathromycin at the concentrations of 5, 10 and 20 μg•mL-1 significantly decreased the production of NO and PGE2, a similar pattern was also observed on the cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS) gene transcription. However, all 5, 10 and 20 μg•mL-1 of erythromycin significantly decreased the production of NO and PGE2 on the transcription doggy diapers and expression level. Conclusion These results support the opinion that macrolides may exert anti-inflammatory effect through modulating the synthesis of NO and PGE2 in inflammatory process.
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